Police said an inquiry into the deaths of two people was extended following the death of an 84-year-old man
All three - including another man aged 71 and a woman, 44 - were patients at Stepping Hill Hospital.
Staff alerted police on Tuesday after finding vials of saline solution had been tampered with.
The discovery came after several patients suffered lower than normal blood sugar levels.
Staff and patients who may have been affected and their relatives have been informed.
The cause of death in all three cases is not yet known.
Greater Manchester Police (GMP) said they had recovered "a number of ampoules of solution" from the hospital and were working closely with the hospital in the investigation.
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